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Updated: Aug 30, 2026

Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
Improved procedure for the determination of malonaldehyde by gas-chromatography with electron-capture detection as
Lorenzo Sangalli1, Luca Maria Chiesa, Elena Passerò
1Department of Veterinary Science and Technology for Food Safety, Faculty of Veterinary Medicine, University of Milan, Via Celoria 10, Milan 20133, Italy.
Abstract:
A previously described derivatization method using trichlorophenylhydrazine was developed for the estimation of malonaldehyde measured by gas-chromatography (GC) with electron-capture detection. The precision and reliability of the procedure are improved here by the use of methylmalonaldehyde as internal standard and by the introduction of a diverter valve at the end of the capillary column to protect the electron-capture detector, respectively. The method was applied to determine malonaldehyde content in bovine plasma samples.
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