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Isolation and characterization of immunoglobulin binding proteins from Staphylococcus intermedius and Staphylococcus

R T Greene¹, C Lämmler

¹Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen.

Zentralblatt Fur Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B

|September 1, 1992

Summary

Staphylococcal IgG binding proteins from S. intermedius and S. hyicus show functional similarity to Staphylococcus aureus protein A. These type I receptors are antigenically similar, suggesting a close relationship between these bacterial species.

Area of Science:

Microbiology
Immunology
Biochemistry

Background:

Staphylococcus intermedius and Staphylococcus hyicus are bacteria that can colonize animals.
Protein A from Staphylococcus aureus is a well-characterized cell surface protein with immunoglobulin G (IgG) binding capabilities.
The presence and characteristics of IgG binding proteins in S. intermedius and S. hyicus are not fully understood.

Purpose of the Study:

To investigate the presence and characteristics of IgG binding proteins on S. intermedius and S. hyicus isolates.
To compare these proteins with protein A from S. aureus.

Main Methods:

Isolating and purifying IgG binding proteins from bacterial cell surfaces.
Testing protein precipitation with various animal IgG preparations and anti-protein A antiserum.
Analyzing protein characteristics using SDS-PAGE, Western blotting, and isoelectric focusing.

Main Results:

IgG binding activity was detected in a high percentage of S. hyicus isolates from pigs and a smaller percentage of S. intermedius isolates from dogs.
Isolated IgG binding proteins from S. intermedius and S. hyicus precipitated with IgG from multiple species (human, rabbit, pig, dog, horse) but not all (cow, mouse, chicken), classifying them as type I receptors.
These proteins showed antigenic similarity to protein A from S. aureus, precipitating with anti-protein A antiserum.
Biochemical analysis revealed similar molecular weights and acidic isoelectric points for the isolated proteins, with slight differences in acidity between species.

Conclusions:

S. intermedius and S. hyicus possess IgG binding proteins that are functionally and antigenically similar to Staphylococcus aureus protein A.
These findings suggest a close relationship between the IgG binding proteins of these staphylococcal species.
The identified proteins represent type I receptors with potential implications in host-pathogen interactions.

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