Related Experiment Videos
Platelet dense granule release reaction monitored by high-performance liquid chromatography-fluorometric
G M Anderson1, L M Hall, J X Yang
1Child Study Center, Yale University School of Medicine, New Haven, Connecticut 06510.
Analytical Biochemistry
|October 1, 1992
Abstract:
The platelet dense granule release reaction was monitored by measuring the serotonin content of platelets collected by filtration following release. Sensitive HPLC-fluorometric analysis permitted release to be assessed using small (25-50 microliters) volumes of plasma and whole blood. Release could be examined for periods as short as 1 s due to the rapid stoppage effected by filtration. The half-life of thrombin-stimulated (1 U/ml) release was 2-3 s, the EC50 values observed for thrombin-stimulated release over a 60-s release period were 0.01-0.03 U/ml for diluted whole blood and plasma samples.