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[ATP-generating creatine kinase: a new compound isolated from Viperidae venom]
D A Kuznetsov1, M A Orlova, A G Berdieva
1Semenov Institute of Chemical Physics, RAS, Moscow, Russia.
Biomeditsinskaia Khimiia
|October 21, 2003
Abstract:
This is a first report on the presence of creatine kinase, ATP-generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin-thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.