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Published on: January 29, 2022
Rapid fibroblast adhesion to 27nm high polymer demixed nano-topography
M J Dalby1, D Giannaras, M O Riehle
1Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow, G12 8QQ, UK. m.dalby@bio.gla.ac.uk
Abstract:
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical method of producing nano-topography. This manuscript investigates human fibroblast response to 27nm high nano-islands produced by polymer demixing. Cell spreading, cytoskeleton, focal adhesion and Rac localisation were studied. The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture.

