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Updated: Aug 30, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
Published on: February 24, 2026
Transverse or axial superresolution in a 4Pi-confocal microscope by phase-only filters
Daniel M de Juana1, José E Oti, Vidal E Canales
1Departamento de Física Aplicada, Universidad de Cantabria, Los Castros S/N, 39005 Santander, Spain.
Abstract:
A novel procedure to design axial and transverse superresolving pupil filters for the 4Pi-confocal microscope is presented. The method is based on the use of a series of figures of merit developed to describe the effect of inserting two identical filters in the two arms of the illumination path of the microscope. As a practical implementation, we have applied our method to obtain superresolving continuous phase-only filters. Different resolution-improving phase functions are shown for the transverse and the axial direction. These filters provided axial gain up to 1.3 and transverse gain up to 1.4 without an increase in sidelobes.
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