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Related Experiment Videos

Extended knobs-into-holes packing in classical and complex coiled-coil assemblies.

John Walshaw1, Derek N Woolfson

  • 1Centre for Biomolecular Design and Drug Development, John Maynard-Smith Building, School of Life Sciences, University of Sussex, Falmer BN1 9QG, UK.

Journal of Structural Biology
|December 4, 2003
PubMed
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Fifty years after Crick's model, new analysis reveals complex coiled-coil structures. Extended "knobs-into-holes" principles explain multi-helix bundles and assemblies beyond simple dimers.

Area of Science:

  • Structural Biology
  • Protein Structure Analysis
  • Bioinformatics

Background:

  • Crick's 1953 paper established the

Purpose of the Study:

  • To extend Crick's principles for understanding complex coiled-coil structures.
  • To develop and apply computational tools for analyzing coiled-coil protein assemblies.

Main Methods:

  • Development of the SOCKET program to identify knobs-into-holes packing.
  • Application of SOCKET to the Protein Data Bank to create a coiled-coil database.
  • Analysis of higher-order oligomers and multi-helix assemblies.

Main Results:

  • The database includes classic coiled coils, 4-helix bundles, and larger helical assemblies.

Related Experiment Videos

  • Complex structures are explained by extending Crick's principles to rings of helices and peripheral knobs-into-holes.
  • Multi-faceted helices, arising from multiple sequence repeats, explain structures beyond dimers.

    • Conclusions:

    • Crick's knobs-into-holes model provides a framework for understanding diverse coiled-coil structures.
    • Higher-order oligomers and complex assemblies can be rationalized by extending core principles.
    • The study provides insights into the relationship between sequence repeats, helix structure, and oligomerization state.