Related Experiment Video
Updated: Aug 30, 2026

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
Published on: July 11, 2011
Improvement of reverse transcription PCR by RNase H
Masao Kitabayashi1, Muneharu Esaka
1Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
Abstract:
An improvement in the method of the Reverse Transcription PCR (RT-PCR) using RNase H is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the Insulin-like growth factor II receptor gene which has the area called the GC-block of about 90% GC contents at the 5' terminal. Furthermore, the RNase H treatment improved the sensitivity of RT-PCR amplification against a general target.
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
RACE - Rapid Amplification of cDNA Ends
Since the...

