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STAT3 is enriched in nuclear bodies.

Andreas Herrmann1, Ulrike Sommer, Albert L Pranada

  • 1Institut für Biochemie, Universitätsklinikum RWTH Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany.

Journal of Cell Science
|December 6, 2003
PubMed
Summary
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Activated STAT3 forms transient nuclear bodies, potentially involved in gene transcription or serving as reservoirs. These STAT3 nuclear bodies associate with active chromatin markers but not PML bodies.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Regulation

Background:

  • Signal transducer and activator of transcription 3 (STAT3) is a crucial transcription factor mediating interleukin-6 (IL-6) signaling.
  • STAT3 activation involves phosphorylation and nuclear translocation to regulate target gene expression.

Purpose of the Study:

  • To investigate the dynamics and characteristics of activated STAT3 within the nucleus.
  • To determine the functional significance of STAT3 nuclear bodies in gene transcription.

Main Methods:

  • Construction of a STAT3-yellow fluorescent protein (YFP) fusion protein for live-cell imaging.
  • Fluorescence recovery after photobleaching (FRAP) to assess STAT3 mobility within nuclear bodies.
  • Colocalization studies with markers of active chromatin (CBP, acetylated histone H4) and PML bodies.

Related Experiment Videos

Main Results:

  • Activated STAT3 forms transient, dot-like structures in the nucleus, termed STAT3 nuclear bodies.
  • STAT3 nuclear bodies exhibit both mobile and immobile fractions, indicating dynamic assembly and disassembly.
  • These bodies colocalize with CREB binding protein (CBP) and acetylated histone H4, suggesting association with transcriptionally active chromatin.

Conclusions:

  • STAT3 nuclear bodies are transient structures formed during IL-6 stimulation.
  • The association with active chromatin markers suggests a role in gene transcription.
  • STAT3 nuclear bodies may function directly in gene transcription or act as reservoirs for activated STAT3.