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Analytical separations in open-tubular capillary electrochromatography
Constantina P Kapnissi-Christodoulou1, Xiaofeng Zhu, Isiah M Warner
1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Electrophoresis
|December 9, 2003
Summary
Open-tubular capillary electrochromatography (OT-CEC) offers an alternative to packed-CEC, immobilizing stationary phases on capillary walls. This review details preparation methods and applications for chiral and achiral separations.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Open-tubular capillary electrochromatography (OT-CEC) presents an alternative to packed capillary electrochromatography (CEC).
- OT-CEC immobilizes the stationary phase onto the inner capillary walls, avoiding issues with frits and silica particles.
Purpose of the Study:
- To review recent advancements in open-tubular capillary electrochromatography (OT-CEC).
- To summarize preparation methods, applications, and challenges of OT-CEC for chiral and achiral separations.
Main Methods:
- The review covers stationary phase immobilization techniques including adsorption, covalent bonding, porous layers, sol-gel, and molecular imprinting.
- It also discusses the coupling of OT-CEC with mass spectrometry (MS).
Main Results:
- Various wall coating preparation methods for OT-CEC columns are detailed.
- Developments, potential applications, technical challenges, and advantages of these methods are presented.
- The review highlights the utility of OT-CEC coupled with MS for analytical separations.
Conclusions:
- OT-CEC provides a viable alternative to packed-CEC with unique advantages for stationary phase immobilization.
- The diverse preparation methods offer flexibility for optimizing chiral and achiral separations.
- OT-CEC, particularly when coupled with MS, shows significant potential in analytical chemistry.