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Updated: Jun 29, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Suppressor current switching: a simple and effective means to reduce background noise in ion chromatography
Panu Rantakokko1, Satu Mustonen, Terttu Vartiainen
1Laboratory of Chemistry, Division of Environmental Health, National Public Health Institute, P.O. Box 95, Kuopio FIN-70701 Finland. panu.rantakokko@ktl.fi
Abstract:
Background noise in ion chromatography with suppressed conductivity detection was significantly reduced for the period of time when the electric current to an anion self regenerating suppressor (ASRS) running in the recycle mode was turned-off. With high capacity AS11-HC columns, it was possible to maintain current free conditions from the beginning of the run past the chloride peak, which enables routine high sensitivity analysis of early to mid eluting peaks. This suppressor current switching was utilized for the analysis of bromate in drinking water with large volume injection using on-line removal of chloride by an On-Guard Ag+-cartridge. The method detection limit (MDL) was 0.21 microg/l in fortified reagent water. Coelution of bromate with an unknown compound was observed, but it was solved by the optimization of gradient program.
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