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Bovine pancreatic kallikrein: purification and radioimmunoassay
A A al-Hamidi1, M A al-Tufail, G S Bailey
1Department of Chemistry and Biological Chemistry, University of Essex, Colchester, UK.
Abstract:
Kallikrein was isolated from bovine pancreatic tissue by a sequential procedure of anion-exchange, hydrophobic interaction and gel filtration chromatographies. A monospecific polyclonal antiserum to the pure enzyme was raised in rabbits and was used to set up a specific radioimmunoassay for bovine tissue kallikrein.