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Intestinal intussusception associated with adenovirus infection in Mexican children

Jeannette Guarner1, Beatriz de Leon-Bojorge, Eduardo Lopez-Corella

  • 1Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop G32, Atlanta, GA 30333, USA.

American Journal of Clinical Pathology
|December 16, 2003
PubMed

Insights

Adenovirus was detected in 33% of pediatric intussusception cases in Mexico using immunohistochemistry and molecular assays. Species C adenovirus, including serotypes 1, 2, and 6, was identified in formalin-fixed intestinal tissues, aiding diagnosis.

Area of Science:

  • Virology
  • Pediatric Gastroenterology
  • Pathology

Background:

  • Intussusception is a common cause of intestinal obstruction in children.
  • The role of viral infections, particularly adenovirus, in pediatric intussusception remains under investigation.
  • Accurate diagnostic methods are crucial for identifying causative agents in formalin-fixed tissues.

Purpose of the Study:

  • To investigate the presence and types of adenovirus in formalin-fixed intestinal tissue from Mexican pediatric patients with intussusception.
  • To evaluate the utility of immunohistochemistry and molecular techniques for adenovirus detection in these specimens.
  • To identify specific adenovirus species and serotypes associated with intussusception.

Main Methods:

  • Analysis of formalin-fixed intestinal tissue from 12 pediatric patients with intussusception.
  • Immunohistochemical staining to detect adenovirus antigen in tissue sections.
  • In situ hybridization using species-specific probes for adenovirus (A-F).
  • Real-time polymerase chain reaction (PCR) for species C adenovirus confirmation and DNA sequencing.

Main Results:

  • Adenovirus antigen was detected in 33% (4/12) of the pediatric intussusception cases.
  • Adenovirus antigens were found in epithelial cells, dendritic cells, and mononuclear inflammatory cells.
  • In situ hybridization confirmed species C adenovirus in 3 patients.
  • Real-time PCR and sequencing identified adenovirus serotypes 1, 2, and 6, with one indeterminate case (2/6).

Conclusions:

  • Species C adenoviruses are present in a subset of Mexican pediatric patients with intussusception.
  • Immunohistochemistry and molecular assays are effective for identifying species C adenoviruses in archival formalin-fixed tissues.
  • This study highlights the potential role of non-enteric adenoviruses in pediatric intussusception.

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