Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T-cell subsets and suppressor cells in human bone marrow.

I G Schmidt-Wolf1, S Dejbakhsh-Jones, N Ginzton

  • 1Department of Medicine, Stanford University School of Medicine, CA.

Blood
|December 15, 1992
PubMed
Summary

Human marrow cells were separated by density. Low-density fractions contain immune suppressor cells and hematopoietic progenitors, suggesting potential for bone marrow transplantation to reduce graft-versus-host disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Opioid Analgesic Reduction Study (OARS) Pilot: A Double-Blind Randomized Multicenter Trial.

JDR clinical and translational research·2023
Same author

Patient mortality following alcohol use and trauma: a propensity-matched analysis.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2017
Same author

A Cost Analysis of Tolerance Induction for Two-Haplotype Match Kidney Transplant Recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Allogeneic hematopoietic cell transplantation after failed autologous transplant for lymphoma using TLI and anti-thymocyte globulin conditioning.

Bone marrow transplantation·2015
Same author

Chimerism, graft survival, and withdrawal of immunosuppressive drugs in HLA matched and mismatched patients after living donor kidney and hematopoietic cell transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Requirement for interactions of natural killer T cells and myeloid-derived suppressor cells for transplantation tolerance.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Human bone marrow contains diverse cell populations with distinct functions.
  • Immune suppression and hematopoiesis are critical processes in bone marrow function.
  • Understanding cell subset distribution is key for therapeutic applications like bone marrow transplantation.

Purpose of the Study:

  • To characterize immune suppressive and hematopoietic features of human marrow cell subsets.
  • To identify specific cell populations responsible for immune suppression and hematopoiesis.
  • To evaluate the potential of marrow cell fractions in allogeneic bone marrow transplantation.

Main Methods:

  • Separation of human marrow cells using Percoll density gradients.
  • Enrichment and purification of cell populations using flow cytometry.

Related Experiment Videos

  • Assessment of immune suppressive activity via mixed leukocyte reaction (MLR).
  • Identification of cell surface markers (CD4, CD8, CD3, CD16) for cell characterization.

    • Main Results:

    • CD4+ and CD8+ T cells were enriched in high-density fractions, while low-density fractions contained suppressor cells and hematopoietic progenitors.
    • CD4-CD8- alpha beta T cells and natural suppressor cells exhibited immune suppressive activity.
    • Low-density fractions suppressed the MLR and showed reduced T cell populations.
    • High-density fractions responded vigorously in the MLR and were depleted of suppressor and progenitor cells.

    Conclusions:

    • Low-density human marrow cell fractions possess both immune suppressor and hematopoietic progenitor activities.
    • These findings suggest potential therapeutic value in allogeneic bone marrow transplantation for reducing graft-versus-host disease.
    • Further investigation of low-density marrow fractions is warranted for optimizing transplantation outcomes.