Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microarray platform comparison for neuroscience applications.

Mark L Parrish1, Nan Wei, Sven Duenwald

  • 1Rosetta Inpharmatics LLC, a wholly owned subsidiary Merck & Co. Inc., Kirkland, WA 98034, USA. mark_parrish@merck.com

Journal of Neuroscience Methods
|December 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Curvature-Modulated Strategy for Single-Atom Catalysts toward Reciprocal Regulation in Li-S Batteries.

Nano letters·2026
Same author

Comparison of three commercial AI tools for detection and malignancy assessment of incidental lung nodules.

Frontiers in medicine·2026
Same author

Attaining 19.12% solar cell efficiency for non-fused ring electron acceptors via multi-dimensional charge transport.

Nature communications·2026
Same author

Noncovalent Macrocyclic Encapsulation via Terminal "Dynamic Locking" Enabling High-Performance Nonfused Ring Electron Acceptors.

Journal of the American Chemical Society·2026
Same author

Green-synthesized ε-polylysine/zein nanocomplexes as safe antimicrobials: Applications and metabolomic mechanisms in grape preservation.

Food chemistry·2026
Same author

CT Radiomics-based Machine Learning to Identify Intratumoral Fibrosis and Underlying Angiogenesis in Hepatocellular Carcinoma.

Radiology·2026
Same journal

Pupil-DLC: an open-source deep learning pipeline for scalable, marker-less tracking of pupil dynamics across conscious and unconscious states.

Journal of neuroscience methods·2026
Same journal

Time as the language of Behavior: events, sequences, patterns and meanings.

Journal of neuroscience methods·2026
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
See all related articles

A novel inkjet microarray system offers superior sensitivity and reproducibility for gene expression profiling of small neural tissue samples (approximately 100 ng total RNA) compared to traditional nylon arrays.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genomics

Background:

  • Gene expression profiling of limited neural tissue samples (approx. 100 ng total RNA) requires highly sensitive methods.
  • Existing methods using nylon cDNA arrays with 33P labeling may lack optimal sensitivity and reproducibility for small samples.

Purpose of the Study:

  • To compare a novel RT-PCR-IVT protocol with fluor-reverse pairs on inkjet oligonucleotide microarrays against a standard RT-IVT protocol using 33P labeling on nylon cDNA arrays.
  • To evaluate the sensitivity, specificity, reproducibility, and linearity of both platforms for analyzing small RNA samples.

Main Methods:

  • Developed a comparative protocol with defined parameters, thresholds, and testing conditions to assess system performance.
  • Utilized inkjet oligonucleotide microarrays with fluor-reverse pairs and nylon cDNA arrays with 33P labeling.

Related Experiment Videos

  • Analyzed approximately 100 ng of total RNA from neural tissue samples.

    • Main Results:

    • The inkjet system demonstrated superior sensitivity, specificity, and reproducibility compared to the nylon array system.
    • Both systems exhibited similar performance in terms of linearity.
    • Established criteria for differentiating system performance across various detection chemistries, instrumentation, probe selection, and sample processing.

    Conclusions:

    • The novel inkjet microarray platform is better suited for transcriptional profiling of small neural tissue samples (approx. 100 ng total RNA) in neuroscience research.
    • This enhanced sensitivity and reproducibility are critical for accurate gene expression analysis in limited biological material.