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Updated: Aug 29, 2026

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
[Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis]
Ping Jiang1, Hongmin Li, Dongjin Chen
1General Hospital of Beijing Coal Mineral Bureau, Beijing 102308, China.
Objective:
To study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease.
Methods:
Mycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains.
Results:
67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal.
Conclusion:
Most of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.
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