Related Experiment Video
Updated: Jul 30, 2026

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Published on: January 8, 2020
NO production by Pseudomonas aeruginosa cd1 nitrite reductase
Francesca Cutruzzolà1, Serena Rinaldo, Fabio Centola
1Dipartimento di Scienze Biochimiche, Universita di Roma La Sapienza, 00185 Rome, Italy. francesca.cutruzzola@uniroma1.it
Abstract:
The structural and catalytic properties of Pseudomonas aeruginosa cd1 nitrite reductase, a key enzyme in bacterial denitrification, are reviewed in this paper. The mechanism of reduction of nitrite to NO is discussed in detail with special attention to the structural interpretation of function. The ability to stabilize negatively charged molecules, such as the substrate (nitrite) and other ligands (hydroxide and cyanide), is a key feature of catalysis in cd1NIRs. The positive potential in the active site is largely due to the presence of the two conserved distal histidines, which are involved in both substrate binding and product release.
Related Concept Videos
Inorganic Nitrogen Assimilation
Gene Regulation in Microbial Communities: Quorum Sensing
Production of Antibiotics

