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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
A nucleobase that releases reporter tags upon DNA oxidation
Akimitsu Okamoto1, Kazuo Tanaka, Isao Saito
1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, and SORST, Japan Science and Technology Corporation, Kyoto 615-8510, Japan.
Abstract:
We have developed a novel nucleosbase, edaG, that efficiently releases various reporter units upon one-electron oxidation. The edaG-selective degradation of ODNs was achieved by various mild oxidizing agents. The oxidant-dependent molecular releasing technique is quite useful not only for DNA-based drug releasing systems but also for the detection of long-range hole transport through DNA without time-consuming analysis.
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