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Updated: Aug 29, 2026
Isolation of Mouse Salivary Gland Stem Cells
Published on: February 8, 2011
Mdm2 mRNA expression in salivary gland tumour cell lines
Andrea Mantesso1, Silvia Vanessa Lourenço Loducca, Israel Bendit
1Department of Oral Pathology, School of Dentistry, University of São Paulo, Av. Lineu Prestes 2227, Cidade Universitária, CEP 05508-900, São Paulo, SP, Brazil. amantesso@hotmail.com
Background:
Murine double minute 2 (mdm2) is a cellular protooncogene, which, in conditions of overexpression or amplification, is capable of inactivating the functions of p53, leading to tumorigenesis. Immunoexpression of mdm2 in salivary gland tumours was previously found; however, it was necessary to find out if mdm2 was overexpressed. The aim of this study was to analyse the mRNA expression of mdm2 in salivary gland neoplasms and to correlate it to immunoexpression of p53 and p21 proteins.
Methods:
Specimens of different salivary gland neoplasms were obtained from surgical resections, and cell lineages derived from these tissues were established. RNA extraction was performed and mRNA expression was investigated using reverse transcription-polymerase chain reaction (RT-PCR). Cellular expression of p53 and p21 proteins was investigated by immunofluorescence technique.
Results:
Increased expression of mdm2 was found in the majority of cell lines analysed.
Conclusions:
Comparing all results, we postulated that overexpression of mdm2 is related to the tumorigenesis and/or tumour progression of salivary gland neoplasms.
Insights
Overexpression of murine double minute 2 (mdm2) was observed in salivary gland neoplasms. This finding suggests mdm2 overexpression is linked to tumor development and progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Murine double minute 2 (mdm2) is a protooncogene implicated in tumorigenesis by inactivating p53.
- Previous studies indicated mdm2 immunoexpression in salivary gland tumors, necessitating further investigation into its overexpression.
- The role of mdm2 in salivary gland neoplasm development requires detailed analysis.
Purpose of the Study:
- To quantify mRNA expression of mdm2 in salivary gland neoplasms.
- To correlate mdm2 mRNA levels with the immunoexpression of p53 and p21 proteins.
- To elucidate the association between mdm2 overexpression and salivary gland tumorigenesis.
Main Methods:
- Surgical resection specimens of salivary gland neoplasms were utilized.
- Cell lineages were established from tumor tissues for analysis.
- Reverse transcription-polymerase chain reaction (RT-PCR) was employed to assess mdm2 mRNA expression.
- Immunofluorescence was used to evaluate p53 and p21 protein expression.
Main Results:
- Elevated mdm2 mRNA expression was detected in most analyzed cell lines derived from salivary gland neoplasms.
- The study identified increased mdm2 expression as a common feature in these tumors.
Conclusions:
- The findings suggest a significant correlation between mdm2 overexpression and the initiation and/or progression of salivary gland neoplasms.
- Overexpression of mdm2 is postulated to play a crucial role in the pathogenesis of these tumors.
