Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Qualitative colorimetric tests for solid phase synthesis.

Jesús Vázquez1, Gregory Qushair, Fernando Albericio

  • 1University of Barcelona, Barcelona Biomedical Research Institute, Barcelona Science Park, Josep Samitier 1, Barcelona, 08028, Spain.

Methods in Enzymology
|January 16, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immobilization of KR-12 on a Titanium Alloy Surface Using Linking Arms Improves Antimicrobial Activity and Supports Osteoblast Cytocompatibility.

ACS applied bio materials·2025
Same author

On-Resin Acetamidomethyl (Acm) Removal and Disulfide Formation in Cysteinyl Peptides Using <i>N</i>-Chlorosuccinimide (NCS) in the Presence of Other Cys-Protecting Groups.

International journal of molecular sciences·2025
Same author

2024 FDA TIDES (Peptides and Oligonucleotides) Harvest.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Base-Labile Safety-Catch Linker: Synthesis and Applications in Solid-Phase Peptide Synthesis.

International journal of molecular sciences·2025
Same author

The Pharmaceutical Industry in 2024: An Analysis of the FDA Drug Approvals from the Perspective of Molecules.

Molecules (Basel, Switzerland)·2025
Same author

Scope and Limitations of the Use of Methanesulfonic Acid (MSA) as a Green Acid for Global Deprotection in Solid-Phase Peptide Synthesis.

ChemSusChem·2025
Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026
Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026
Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026
Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026
Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026
Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026
See all related articles

Colorimetric tests in synthesis require careful execution. Ensuring reagent purity, immediate testing after synthesis steps, and using controls are crucial for accurate results and reproducibility.

Area of Science:

  • Synthetic Chemistry
  • Analytical Chemistry

Background:

  • Colorimetric tests are widely used for monitoring synthetic reactions.
  • Variations in reproducibility and accuracy have been observed in these tests.

Purpose of the Study:

  • To highlight critical factors affecting colorimetric test results.
  • To provide recommendations for improving the reliability of colorimetric assays in synthesis.

Main Methods:

  • Summarized results from applied colorimetric tests (Table II).
  • Identified factors influencing test outcomes: reagent stability, resin type, functional group interference, protecting group lability.

Main Results:

  • Observed variations in test reproducibility and accuracy.
  • Highlighted the impact of reagent purity and prompt testing on results.

Related Experiment Videos

  • Emphasized the risk of false positives/negatives due to resin-product bond lability or product degradation.

    • Conclusions:

    • Recommend performing positive and negative controls for new syntheses.
    • Advise using pure, properly stored reagents and testing immediately after synthesis completion.
    • Suggest repeating tests for ambiguous results and using multiple tests for complex cases.