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Genetic instability in EBV-transformed lymphoblastoid cell lines
Aisha Mohyuddin1, Qasim Ayub, Saima Siddiqi
1Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories, G.P.O. Box 2891, Islamabad 44000, Pakistan.
Biochimica Et Biophysica Acta
|January 20, 2004
Summary
Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines offer a stable DNA source. Microsatellite instability analysis revealed a low somatic mutation rate of 0.3% in these commonly used cell lines.
Area of Science:
- Genetics
- Molecular Biology
- Virology
Background:
- Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) are widely utilized for DNA extraction.
- These cell lines are crucial for various genetic and molecular studies.
- Assessing genomic stability in LCLs is important for data interpretation.
Purpose of the Study:
- To quantify microsatellite instability in EBV-transformed lymphoblastoid cell lines.
- To determine the rate of somatic mutations in LCLs compared to original blood samples.
- To establish the reliability of LCLs as a DNA source for mutation studies.
Main Methods:
- DNA was collected from 35 individuals, including original blood samples and EBV-transformed lymphoblastoid cell lines.
- Microsatellite instability analysis was performed on DNA from both sources.
- Somatic mutation rates were calculated based on observed differences.
Main Results:
- Microsatellite instability was assessed in parallel samples from blood and cultured cell lines.
- Mutations were detected in 0.3% of the microsatellite analyses performed.
- This finding provides a quantitative measure of the somatic mutation rate in LCLs.
Conclusions:
- EBV-transformed lymphoblastoid cell lines exhibit a low rate of microsatellite instability.
- The observed mutation rate of 0.3% validates LCLs as a reliable source of DNA for genetic studies.
- These findings support the continued use of LCLs in research requiring stable DNA templates.