Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A methylation-mediator complex in hormone signaling.

Wei Xu1, Helen Cho, Shilpa Kadam

  • 1Howard Hughes Medical Institute, Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Genes & Development
|January 20, 2004
PubMed
Summary

Nuclear hormone receptors recruit coactivators to promote transcription. This study identifies a new complex linking the methylase CARM1 and the SWI/SNF remodeling complex, revealing a novel regulatory mechanism in nuclear hormone signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a Rapid-Acting Oxidized Regenerated Cellulose-Based Powder for Hemostatic Applications.

ACS omega·2026
Same author

Development and Evaluation of Bilayer Sustained-Release Tablets of Ruxolitinib Using Discriminative Pharmacokinetic Analysis and IVIVC.

Pharmaceutics·2025
Same author

Biodegradability and Efficacy of Porous Polycaprolactone Microsphere Dermal Filler for Fine Lines.

Journal of cosmetic dermatology·2025
Same author

Development of a PLA Polymer-Based Liquid Filler for Next-Generation Aesthetics.

International journal of molecular sciences·2025
Same author

First-in-human, phase 1 study of PF-06753512, a vaccine-based immunotherapy regimen (VBIR), in non-metastatic hormone-sensitive biochemical recurrence and metastatic castration-resistant prostate cancer (mCRPC).

Journal for immunotherapy of cancer·2023
Same author

Preclinical development of a vaccine-based immunotherapy regimen (VBIR) that induces potent and durable T cell responses to tumor-associated self-antigens.

Cancer immunology, immunotherapy : CII·2022

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Nuclear hormone receptors (NRs) regulate transcription by recruiting coactivators.
  • Histone methylation enzymes like CARM1 and ATP-remodeling complexes are individually linked to NR-dependent transcription.
  • A functional link between these systems was previously unidentified.

Purpose of the Study:

  • To investigate the functional and mechanistic linkage between CARM1 and ATP-remodeling complexes in nuclear receptor signaling.
  • To identify and characterize novel coactivator complexes involved in NR-dependent transcription.

Main Methods:

  • Purification of endogenous CARM1-interacting proteins.
  • Identification of the nucleosomal methylation activator complex (NUMAC) using biochemical approaches.

Related Experiment Videos

  • In vivo studies examining the coassembly of CARM1 and BRG1 on ER-target genes.

    • Main Results:

    • The nucleosomal methylation activator complex (NUMAC) was identified, containing CARM1 and SWI/SNF components including BRG1.
    • CARM1 within NUMAC specifically methylates nucleosomal histones and enhances its activity.
    • CARM1 reciprocally stimulates the ATPase activity of BRG1, and they coassemble on ER target genes in vivo.

    Conclusions:

    • The association of CARM1 with the SWI/SNF ATP-remodeling complex (NUMAC) defines a new regulatory component in nuclear hormone signaling.
    • This complex cooperatively activates estrogen receptor-dependent transcription.
    • This finding establishes a mechanistic link between histone methylation and nucleosome remodeling in gene activation.