Related Experiment Videos
[Comparative study of lipophilicity from immobilized artificial chromatography and n-octanol/buffer systems]
Jin Sun1, Gang Cheng, Zhong-gui He
1Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. sunjin66@21cn.com
Aim:
To compare lipophilicity measuring scale stemmed from immobilized artificial membrane chromatography and n-octanol/buffer systems.
Methods:
A test set consisted of 27 structurally diverse compounds. The lipophilicity of these were evaluated by both immobilized artificial membrane chromatography (IAMC) and n-octanol/buffer systems, which were expressed as lg kIAM and lg DO/W,7.4, respectively.
Results:
With regard to each individual group, good correlation coefficient (r2) over 0.81 was obtained (0.82 for acid; 0.88 for neutral, 0.81 for base and 0.92 for ampholyte, respectively). However, a smaller r2 (0.62) was acquired for all compounds studied than that of each individual group.
Conclusion:
IAMC and n-octanol/buffer systems were shown to be different in lipophilicity.