[Evaluation of interaction between drugs and ordered phospholipid membrane by immobilized artificial membrane
Jin Sun1, Gang Cheng, Zhong-gui He
1Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. sunjin66@21cn.com
Aim:
To investigate the interaction between drugs and ordered phospholipid membrane using immobilized artificial membrane chromatography (IAMC).
Methods:
IAMC was used to determine the interaction drugs with phospholipid membrane, expressed as membrane affinity (lg kIAM). An n-octanol/buffer system was also employed as the reference hydrophobicity (lg Do/w,7.4).
Results:
Within the range of used acetonitrile percentages (phi) 0-30% in mobile phase, retention index (lg kIAM) showed excellent correlation with phi. Intercepts of fitted straight lines between lg kIAM and phi were comparable but slopes were much different for the three organic modifiers (acetonitrile, ethanol and methanol). Effects by adding CH2 substituent on lipophilicity difference (delta lg kIAM and delta lg Do/w,7.4) were similar for p-hydroxyl benzoic methyl ester to butyl ester, whereas different for p-hydroxylbenzoic acid to methyl ester.
Conclusion:
IAMC system is a convenient, efficient and rapid tool for determining membrane interaction.
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