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In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
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In vivo antigen presentation

Ronald N Germain1, Marc K Jenkins

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Drive, MSC-1892, Bethesda, MD 20892-1892, USA. RGERMAIN@niaid.nih.gov

Current Opinion in Immunology
|January 22, 2004
PubMed

Monoclonal antibodies specific for defined peptide-MHC complexes are now being used to physically detect T-cell receptor ligands. These reagents have resulted in the identification of the cells that present antigen in lymphoid and non-lymphoid tissues after various forms of antigen administration. In addition, recent advances in real-time imaging technology have begun to measure the rate and directionality of T-cell movement relative to antigen-presenting cells in lymph nodes, shedding light on the earliest events in T-cell activation in a physiological setting.

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