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Enteropathogenic activity and invasion of HEp-2 cells by Aeromonas caviae clinical isolates

Sylwia Krzymińska1, Adam Kaznowski, Karolina Lindner

  • 1Department of Microbiology, A. Mickiewicz University Fredry 10, 61-701 Poznań, Poland.

Acta Microbiologica Polonica
|January 28, 2004
PubMed

Insights

Aeromonas caviae strains from children with diarrhea exhibit virulence factors. Most isolates produced cytotoxic and cytotonic toxins, with some showing invasive capabilities in cell models.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Bacterial Pathogenesis

Background:

  • Aeromonas species are emerging pathogens causing gastrointestinal infections.
  • Diarrhea in children is a significant public health concern, often linked to bacterial infections.
  • Understanding the virulence factors of bacterial isolates is crucial for disease management.

Purpose of the Study:

  • To investigate the virulence-associated properties of Aeromonas caviae isolates from pediatric diarrhea cases.
  • To determine the prevalence of toxin production (cytotoxic and cytotonic) and invasive potential in these strains.

Main Methods:

  • Twenty Aeromonas caviae isolates were obtained from stool samples of children with diarrhea.
  • Virulence factors assessed included cytotoxic and cytotonic toxin production using cell lines (Vero and CHO).
  • Invasive ability was evaluated using HEp-2 cell monolayers.

Main Results:

  • The majority of Aeromonas caviae isolates demonstrated cytotoxic effects on Vero and CHO cells.
  • Cytotonic toxins, leading to CHO cell elongation, were produced by most strains.
  • Invasive potential was observed in five (25%) of the examined Aeromonas caviae isolates against HEp-2 cells.

Conclusions:

  • Aeromonas caviae possesses significant virulence factors, including toxin production and invasive capacity, contributing to pediatric diarrhea.
  • These findings highlight the pathogenic potential of Aeromonas caviae in children.
  • Further research into Aeromonas-associated gastroenteritis is warranted.

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