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Recombination in uveitis-causing enterovirus strains.
A N Lukashev1,2, V A Lashkevich2, G A Koroleva2
1Department of Biochemistry and Pharmacy, Åbo Akademi University, PO Box 66, FIN-20521 Turku, Finland.
The Journal of General Virology
|February 11, 2004
Summary
Sequencing of echovirus (EV) strains revealed mosaic recombinants responsible for enterovirus uveitis (EU) outbreaks. These findings suggest independent emergence of EU-causing strains rather than recent recombination events.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Enterovirus uveitis (EU) is an infant disease first identified in Siberia in 1980.
- Echovirus (EV) outbreaks have been linked to EU and sepsis-like illnesses.
Purpose of the Study:
- To determine the complete nucleotide sequences of human echovirus (EV) 11 and EV19 strains.
- To investigate the genetic basis and emergence of enterovirus uveitis (EU)-causing strains.
Main Methods:
- Whole genome sequencing of four EV11 strains and one EV19 strain.
- Comparative sequence analysis of viral genomes and non-structural coding regions.
Main Results:
- Three EV11 strains causing EU outbreaks were mosaic recombinants of the prototype EV11 Gregory strain.
- Non-structural coding regions and 5' NTRs showed similarity to other enteroviruses (EV1, EV9).
- Full genome comparison excluded recent recombination as the cause for the emergence of EU-causing strains, indicating independent emergence.
Conclusions:
- The genetic makeup of circulating enteroviruses may not directly correlate with altered virulence.
- EU-causing echovirus strains emerged independently, not through recent recombination events.