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Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation
Published on: March 14, 2017
[The relationship between morphology and cell activity in hyperparathyroidism]
1Department of Urology, Kinki University School of Medicine.
Abstract:
The relationship between tissue morphology and cellular activity in hyperparathyroidism was investigated by 2-color flowcytometry and an immunohistochemical technique using anti-parathyroid hormone (PTH) antibody. A total of 21 cases were employed, which included 8 cases of primary hyperparathyroidism (PHPT) and 13 cases of secondary hyperparathyroidism (SHPT). The results of 2-color flowcytometry on DNA ploidy pattern revealed that all the 8 PHPT cases were diploids. In SHPT, however, there were 9 diploids (69.2%), 2 tetraploids (15.4%) and 2 aneuploids (15.4%). Electron microscopic studies were performed on the diploid 2c and 4c peak cells, which were obtained by individually sorting the cells from each cycle. As a result, these cells were found to possess well developed rough endoplasmic reticulum and Golgi apparatus. These cells in general showed weak immunostaining. Furthermore, numerous secretory granules were found in the cells obtained from abnormal peak of aneuploid when compared to diploid cells. In tetraploids and aneuploids, the darkly stained cells and glandular cells showed intense immunostaining. These cells contained numerous secretory granules and they were thought to possess high cellular activity.
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