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Adsorption of nafamostat mesilate by hemodialysis membranes

O Inagaki1, Y Nishian, R Iwaki

  • 1Department of Kidney and Dialysis, Hyogo College of Medicine, Nishinomiya, Japan.

Artificial Organs
|December 1, 1992
PubMed

Insights

Nafamostat mesilate (FUT-175) strongly adsorbs to polyacrylonitrile (AN69) hemodialysis membranes, reducing its anticoagulant effectiveness. This adsorption, driven by ionic bonds, makes FUT-175 unsuitable for AN69 membranes.

Area of Science:

  • Biomaterials Science
  • Nephrology
  • Pharmacology

Background:

  • Nafamostat mesilate (FUT-175) is an anticoagulant used during hemodialysis.
  • Hemodialysis membranes can interact with and adsorb drugs, potentially affecting their efficacy.
  • Understanding drug-membrane interactions is crucial for optimizing anticoagulation therapy.

Purpose of the Study:

  • To investigate the adsorption of nafamostat mesilate (FUT-175) by various hemodialysis membranes.
  • To determine the impact of FUT-175 adsorption on its anticoagulant activity in vivo and in vitro.
  • To elucidate the mechanism of FUT-175 adsorption by hemodialysis membranes.

Main Methods:

  • In vivo and in vitro adsorption studies of FUT-175 with five hemodialysis membranes (AN69, J-PAN, Cuprophan, Hemophan, PMMA).
  • Measurement of celite-activated coagulation time in patients undergoing hemodialysis with FUT-175.
  • Staining of membranes with methylene blue to assess charge characteristics.

Main Results:

  • FUT-175 was strongly adsorbed by the polyacrylonitrile (AN69) membrane and slightly by the J-PAN membrane in vivo and in vitro.
  • No significant adsorption of FUT-175 was observed with Cuprophan (CU), Hemophan (HE), or polymethylmethacrylate (PMMA) membranes.
  • FUT-175 did not prolong celite-activated coagulation time during hemodialysis with the AN69 membrane.
  • Methylene blue staining indicated that AN69, J-PAN, and PMMA membranes possess negative charges.

Conclusions:

  • Nafamostat mesilate (FUT-175) adsorbs to negatively charged hemodialysis membranes, such as AN69, J-PAN, and PMMA, via ionic bonding.
  • The strong adsorption of FUT-175 by the AN69 membrane compromises its anticoagulant function.
  • FUT-175 is unsuitable for use as an anticoagulant with AN69 hemodialysis membranes due to significant drug adsorption.

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