Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell differentiation and patterning in Dictyostelium.

R R Kay1

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Current Opinion in Cell Biology
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The genome of the social amoeba Dictyostelium discoideum.

Nature·2005
Same author

Cross-induction of cell types in Dictyostelium: evidence that DIF-1 is made by prespore cells.

Development (Cambridge, England)·2001
Same author

The role of DIF-1 signaling in Dictyostelium development.

Molecular cell·2001
Same author

Cell-fate choice in Dictyostelium: intrinsic biases modulate sensitivity to DIF signaling.

Developmental biology·2000
Same author

Ca(2+) signalling is not required for chemotaxis in Dictyostelium.

The EMBO journal·2000
Same author

Development at the edge of multi-cellularity: Dictyostelium discoideum.

The International journal of developmental biology·2000
Same journal

Mechanosensing in immune cells: Implications for migration and beyond.

Current opinion in cell biology·2026
Same journal

Emerging role of organelles in cell migration.

Current opinion in cell biology·2026
Same journal

Nuclear adaptation in cell migration.

Current opinion in cell biology·2026
Same journal

Patterns in motion: Choreographing dynamic cell behaviours during tissue repair.

Current opinion in cell biology·2026
Same journal

Quo vadis reconstituted cell surfaces? Purpose and future perspectives for minimal systems of the cell plasma membrane.

Current opinion in cell biology·2026
Same journal

Nuclear determinants of mRNA and protein isoforms.

Current opinion in cell biology·2026
See all related articles

Dictyostelium development involves cell sorting and differentiation regulated by chemical signals like cAMP and DIF-1. New genetic techniques will aid in isolating key developmental genes.

Area of Science:

  • Cellular and Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Dictyostelium discoideum serves as a model organism for studying cellular differentiation and morphogenesis.
  • Prestalk cells differentiate and sort within multicellular aggregates, guided by chemoattractant gradients.

Purpose of the Study:

  • To elucidate the mechanisms regulating cell differentiation and proportion control in Dictyostelium.
  • To understand the role of signaling molecules, such as cAMP and DIF-1, in Dictyostelium development.
  • To introduce novel genetic tools for identifying developmental genes.

Main Methods:

  • Observation of cell differentiation and sorting in Dictyostelium aggregates.
  • Analysis of signaling pathways involving cyclic adenosine monophosphate (cAMP) and differentiation-inducing factor-1 (DIF-1).

Related Experiment Videos

  • Utilizing insertional mutagenesis for gene discovery.

    • Main Results:

    • Prestalk cell differentiation and sorting are likely mediated by chemotaxis to cAMP.
    • Cell proportion regulation may involve selective depletion of the stalk cell inducer, DIF-1.
    • DIF-1 gradients are established in the slug, influencing cell behavior.
    • Terminal differentiation requires activation of cAMP-dependent protein kinase, potentially linked to ammonia depletion.

    Conclusions:

    • Cellular differentiation and spatial organization in Dictyostelium are tightly regulated by extracellular signals.
    • The study highlights the interplay between signaling molecules and cellular processes in development.
    • Insertional mutagenesis offers a powerful approach for future genetic studies in Dictyostelium development.