Related Experiment Videos
Construction of a shuttle lacZ alpha-based Escherichia coli-actinomycetes vector containing the phage JHJ-3 replicon
L R Gaudreau1, C Le Gouill, C V Déry
1Département de Biologie, Université de Sherbrooke, Québec, Canada.
FEMS Microbiology Letters
|December 1, 1992
Abstract:
Using the broad replicating range JHJ-3 phage replicon, a shuttle vector for Escherichia coli and actinomycetes has been constructed. The vector, pOJ31, bears the lacZ alpha fragment allowing a blue/white gene cloning system. pOJ31 also contains a polylinker of 15 unique cloning sites and the phage T7 promoter. The vector has been used to stably express the mel gene from plasmid pIJ702 in Streptomyces lividans.