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High-performance liquid chromatographic determination of cyanide in human red blood cells by pre-column fluorescence
A Sano1, N Takimoto, S Takitani
1Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Journal of Chromatography
|November 6, 1992
Abstract:
A method for the determination of cyanide in human red cells has been developed. Cyanide was extracted from red cells by adding water and methanol, and then derivatized with 2,3-naphthalene-dialdehyde and taurine to give a fluorescent product, which was determined by reversed-phase high-performance liquid chromatography with fluorescence detection. The recovery of cyanide from red cells was ca. 83%, and the limit of detection was 100 pmol/ml. The mean concentrations of red cell cyanide from ten smokers and from ten non-smokers were 705 and 466 pmol/ml, respectively. The method was also applicable to whole blood.