Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rate-limiting components and reaction steps in complement-mediated haemolysis.

H E Nielsen1, S O Larsen, T Vikingsdottir

  • 1Complement Laboratory, Statens Seruminstitut, Copenhagen, Denmark.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activation of Complement Following Total Hip Replacement.

Scandinavian journal of immunology·2016
Same author

Increased risk of preterm delivery and pre-eclampsia in women with polycystic ovary syndrome and hyperandrogenaemia.

BJOG : an international journal of obstetrics and gynaecology·2014
Same author

International Standard for Antistreptolysin-O.

Bulletin of the World Health Organization·2010
Same author

Do non-steroidal anti-inflammatory drugs influence chronic inflammation? The effects of piroxicam on chronic antigen-induced arthritis in rats.

Scandinavian journal of rheumatology·2008
Same author

Setting truncation limits for marker values in medical screening.

Journal of medical screening·2007
Same author

First trimester Down syndrome screening: distribution of markers and comparison of assays for quantification of pregnancy-associated plasma protein-A.

Scandinavian journal of clinical and laboratory investigation·2006
Same journal

Immune Cell Composition and Prognosis in Node-Positive, Irradiated Breast Cancer Patients in the DBCG-IMN2 Study.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

Urinary Secretory-IgA and IgG Responses and Inflammatory Markers in Patients With Pseudomonas aeruginosa Urinary Tract Infections.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

Predictive Immunohistochemistry in Cholangiocarcinoma: Current Clinical Utility, Practical Limitations, and Emerging Directions.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

Active Chlorine Antiseptics in Wound Care: Recent Advances and Perspectives for Military Medicine.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

A Review on the Logistics, Financial, Ethical, and Regulatory Frameworks of Artificial Intelligence in Digital Pathology.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same journal

UE, CT, and ACR TI-RADS Grading in the Combined Diagnosis of Benign and Malignant Thyroid Nodules.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
See all related articles

Identifying rate-limiting steps in complement system activation is crucial. This study found multiple components limit both alternative and classical pathways, with specific steps identified in the classical pathway.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • The complement system comprises distinct activation pathways (alternative and classical) crucial for immune response.
  • Understanding rate-limiting steps is key to modulating complement system activity.

Purpose of the Study:

  • To identify rate-limiting components and reaction steps in the alternative pathway (AP) and classical pathway (CP) of the complement (C) system.
  • To elucidate the integrated activation sequences of the AP and CP.

Main Methods:

  • Correlation analysis of haemolysis rates with component concentrations.
  • Addition of purified complement components and IgM to normal human serum to assess impact on haemolysis.
  • Reconstitution experiments to pinpoint rate-limiting reaction steps.

Related Experiment Videos

Main Results:

  • Haemolysis rates in AP correlated with C5 and IgM concentrations.
  • Haemolysis rates in CP correlated with C2-C6, factors I and B, and IgM concentrations.
  • Multiple components, not a single one, were found to be rate-limiting in both AP and CP.
  • In CP, C4 and C2 activation were identified as rate-limiting steps.
  • In AP, the rate-limiting step occurs at or before C3 activation.

Conclusions:

  • The complement system's alternative and classical pathways are complex, with multiple rate-limiting factors.
  • Specific steps in classical pathway activation (C4 and C2) are critical.
  • Further investigation is needed to precisely define rate-limiting steps in the alternative pathway before C3 activation.