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Related Experiment Videos

Cytokine flow cytometry.

Holden T Maecker1

  • 1Immunocytometry Systems, BD Biosciences, San Jose, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2004
PubMed
Summary
This summary is machine-generated.

Cytokine flow cytometry (CFC) enables the quantification of rare antigen-specific T cells by analyzing intracellular cytokine production. This method involves fixed and permeabilized cells after in vitro activation, crucial for immune response studies.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Cytokine flow cytometry (CFC) is a flow cytometry technique utilizing anticytokine antibodies.
  • Intracellular cytokine staining after cell fixation and permeabilization is a common CFC method.
  • This technique is vital for quantifying rare antigen-specific T cells.

Purpose of the Study:

  • To elaborate on the specific methodology for intracellular cytokine staining.
  • To emphasize the impact of various factors on the technique's outcomes.
  • To provide a comprehensive guide for researchers using CFC.

Main Methods:

  • Flow cytometry analysis using anticytokine antibodies.
  • Intracellular staining of cytokines in fixed and permeabilized cells.

Related Experiment Videos

  • In vitro activation of cells with specific antigens.

    • Main Results:

    • The methodology allows for the accurate quantitation of rare antigen-specific T cell populations.
    • Optimization of sample type, antigens, activation conditions, and processing is critical.
    • Effective data acquisition and analysis are essential for reliable results.

    Conclusions:

    • Cytokine flow cytometry is a powerful tool for immune cell analysis.
    • Understanding methodological variables is key to successful CFC application.
    • This technique significantly advances the study of cellular immune responses.