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Myofibers express IL-6 after eccentric exercise
Akihito Tomiya1, Toshimi Aizawa, Ryoichi Nagatomi
1Department of Orthopaedic Surgery, Tohoku University School of Medicine, Sendai, Japan.
The American Journal of Sports Medicine
|February 24, 2004
Summary
Interleukin-6 (IL-6) expression rises in muscle fibers after strenuous eccentric exercises, preceding muscle damage. This suggests IL-6 plays a key role in exercise-induced muscle injury and repair processes.
Area of Science:
- Exercise physiology
- Muscle biology
- Immunology
Background:
- Interleukin-6 (IL-6) is produced in skeletal muscle.
- Plasma IL-6 levels significantly increase following eccentric exercise.
Purpose of the Study:
- Identify muscle cell types responsible for IL-6 production post-eccentric exercise.
- Investigate the temporal expression and localization of IL-6.
Main Methods:
- Eccentric contraction model induced by electrical stimulation.
- Histological analysis of muscle damage and regeneration.
- Immunohistochemical detection of IL-6 expression and localization in muscle cells.
Main Results:
- Myofiber swelling observed 8 hours to 3 days post-exercise; disruption peaked at 3 days.
- IL-6 initially localized in myofiber cytoplasm, then in inflammatory and satellite cells.
- Myofiber IL-6 expression increased immediately post-exercise, peaking at 12 hours.
Conclusions:
- IL-6 expression in myofibers increases before myofiber disruption after eccentric exercise.
- IL-6 is strongly associated with muscle damage resulting from strenuous exercise.