Related Experiment Videos
Characterization of citrus HSVd isolates.
A Palacio-Bielsa1, J Romero-Durbán, N Duran-Vila
1Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Spain.
Archives of Virology
|March 3, 2004
Summary
Hop stunt viroid (HSVd) variability was analyzed using sequencing. Specific nucleotide differences in the V domain distinguish cachexia-inducing HSVd strains, impacting molecular structure and stability.
Area of Science:
- Plant virology
- Molecular biology
- Genetics
Background:
- Hop stunt viroid (HSVd) causes disease in citrus.
- Understanding HSVd variability is crucial for disease management.
Purpose of the Study:
- To investigate the genetic variability of HSVd isolates from citrus.
- To identify molecular markers associated with cachexia induction by HSVd.
Main Methods:
- Retrotranscription and DNA amplification (RT-PCR) of HSVd isolates.
- Single-strand conformation polymorphism (SSCP) analysis for variability detection.
- DNA sequencing of HSVd variants, focusing on the V domain.
Main Results:
- SSCP analysis revealed variability within and among HSVd sources.
- Electrophoretic profiles differentiated cachexia and non-cachexia HSVd sources.
- Five nucleotide differences in the V domain were identified as a cachexia-discriminating motif.
- Cachexia HSVd variants exhibited lower conformational stability compared to non-cachexia variants.
Conclusions:
- Specific nucleotide variations in the HSVd V domain are linked to cachexia induction.
- These variations likely alter the viroid's three-dimensional structure and stability.
- Host effects influence the evolution and variability of viroid quasispecies.