Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Surface-functionalized nanoparticle library yields probes for apoptotic cells.

Eyk A Schellenberger¹, Fred Reynolds, Ralph Weissleder

¹Harvard Medical School, Center for Molecular Imaging Research, Building 149, 13th Street, 5403, Charlestown, MA 02129, USA.

Chembiochem : a European Journal of Chemical Biology

|March 5, 2004

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decoration of Autophagy Detecting Nanoparticle with an Anionic Fluorochrome Enhances Multispectral Characterization of Autophagosome Location and Flux.

Small (Weinheim an der Bergstrasse, Germany)·2024

Same author

Preclinical Evaluation of Novel Positron Emission Tomography (PET) Probes for Imaging Leucine-Rich Repeat Kinase 2 (LRRK2).

Journal of medicinal chemistry·2024

Same author

Radiochemistry for positron emission tomography.

Nature communications·2023

Same author

Imaging Leucine-Rich Repeat Kinase 2 In Vivo with <sup>18</sup>F-Labeled Positron Emission Tomography Ligand.

Journal of medicinal chemistry·2022

Same author

A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence.

Nature biomedical engineering·2022

Same author

A novel monoacylglycerol lipase-targeted <sup>18</sup>F-labeled probe for positron emission tomography imaging of brown adipose tissue in the energy network.

Acta pharmacologica Sinica·2022

Same journal

Sustainable Synthesis of Bio-Based Furanic and Aromatic Amines Using an Optimized Whole-Cell Transaminase-Decarboxylase Cascade in E. coli RARE.

Chembiochem : a European journal of chemical biology·2026

Same journal

Composite Dissolvable Microneedle-Enabled Local High-Dose Delivery of Endogenous Thiols for Efficient Allergic Dermatitis Treatment.

Chembiochem : a European journal of chemical biology·2026

Same journal

Revisiting the Radical Quenching Activity of Ebselen Analogues in Homogeneous Phase and In Silico Anti-ferroptotic Activity with Histone Deacetylase.

Chembiochem : a European journal of chemical biology·2026

Same journal

Reaction Optimization for Enzymatic Deconstruction of Industrially Relevant Nylon Composites.

Chembiochem : a European journal of chemical biology·2026

Same journal

Deploying Artificial Metalloenzymes in Complex Environments: Strategies and Applications.

Chembiochem : a European journal of chemical biology·2026

Same journal

Synthetic Ligands of Myeloid C-Type Lectin Receptors.

Chembiochem : a European journal of chemical biology·2026

See all related articles

Researchers created surface-functionalized nanoparticles that can identify specific cells, like apoptotic Jurkat cells, similar to annexin V. This technology enables targeted cell recognition for various applications, including drug delivery and imaging.

Area of Science:

Biomaterials Science
Nanotechnology
Cell Biology

Background:

Developing targeted nanomaterials for specific cell recognition is crucial for advanced diagnostics and therapeutics.
Existing methods for cell targeting can be limited in specificity or detection capabilities.

Purpose of the Study:

To develop and validate surface-functionalized nanoparticles for the selective recognition of specific cell types.
To demonstrate the utility of these nanoparticles in distinguishing between normal and apoptotic cells.

Main Methods:

Efficient synthesis and screening of nanoparticle libraries.
Surface functionalization of nanoparticles for targeted cell interaction.
Detection and analysis using fluorescence-activated cell sorting (FACS) and fluorescence microscopy.

Related Experiment Videos

Utilizing NMR relaxometry for nanoparticle detection.

Main Results:

Developed nanoparticles that preferentially recognize apoptotic Jurkat cells, mimicking the function of annexin V.
Demonstrated successful detection of targeted cells using fluorescence and NMR relaxometry.
Validated nanoparticle specificity through FACS analysis and fluorescence microscopy.

Conclusions:

Surface-functionalized nanoparticles can be efficiently synthesized and screened for specific cell recognition.
This approach offers a versatile platform for developing targeted nanomaterials.
Potential applications include advanced imaging agents (quantum dots, colloidal gold) and drug delivery systems.