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The osteoblast mechano-receptor, microgravity perception and thermodynamics
Erk Klopp1, Daniel Graff, Jens Struckmeier
1Department of Semiconductor Physics, University of Marburg, Marburg, Germany. Erk.Klopp@physik.uni-marburg.de
Journal of Gravitational Physiology : a Journal of the International Society for Gravitational Physiology
|March 9, 2004
Summary
Microgravity does not affect intracellular calcium or gene expression in osteoblasts. Cellular mechanical and thermal noise mask the subtle force changes, requiring high forces for cell activation.
Area of Science:
- Cell Biology
- Biophysics
- Space Biology
Background:
- Cellular mechano-sensing is crucial, involving intracellular free calcium (IFC), gene regulation, and second messenger systems.
- Osteoblasts, as non-professional cells, possess mechano-sensing capabilities that warrant investigation under altered gravity conditions.
Purpose of the Study:
- To determine if osteoblasts can detect microgravity via their mechano-sensors.
- To analyze the impact of microgravity on intracellular free calcium (IFC) and gene expression in osteoblasts.
Main Methods:
- Experiments conducted during sub-orbital rocket flights and parabolic flights.
- Measurements of intracellular free calcium (IFC) and gene expression.
- Complementary substrate force measurements on osteoblasts.
Main Results:
- Microgravity exposure did not significantly alter IFC levels in osteoblasts.
- Gene expression patterns remained unchanged in osteoblasts under microgravity.
- Cells generate substantial forces on their substrate, and high forces are necessary for activation.
Conclusions:
- The subtle force changes associated with microgravity are masked by significant intracellular thermal and mechanical noise.
- Osteoblasts do not appear to detect or respond to microgravity through their known mechano-sensing pathways under these experimental conditions.