Related Experiment Video
Updated: Aug 25, 2026
TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
Published on: September 10, 2015
Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations
Olga O Sineshchekova1, Toshimitsu Kawate, Oleksandr V Vdovychenko
1The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA. Olga.Sineshchekova@UTSouthwestern.edu
Background:
"Protein-trap" is a method that allows epitope-tagging of endogenous proteins. This method allows for the identification of endogenously expressed proteins that exhibit specific localization of interest. This method has been recently reported for its application in the study of Drosophila development by using a relatively large epitope, green-fluorescent-protein (GFP).
Result:
Herein, we report a new "protein-trap" vector for mammalian cells. This new method utilizes a much smaller epitope-tag and also allows for drug-selection prior to the epitope-tagging. Pre-selection by drug resulted in the highly efficient protein-trapping frequency.
Conclusion:
The "protein-trap" method based on this new vector is expected to serve as a complimentary approach to the previously reported GFP-based method.
More Related Videos
12:30Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
Published on: March 5, 2012
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019