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Updated: Aug 18, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform
Published on: August 23, 2012
Generation of protein in situ arrays by DiscernArray technology
1Discerna Limited, Cambridge, UK.
Abstract:
A cell-free DiscernArray technology is described for making protein arrays directly from polymerase chain reaction deoxyribonucleic acid (PCR DNA) fragments. In this method, individual tagged proteins are synthesized in a cell-free system on a tag-binding surface, such that the tagged proteins are immobilized on the surface as they are produced. This technology is particularly useful for arraying proteins/domains that cannot be functionally produced by heterologous expression or for which the cloned DNA is not available. Protein arrays of single-chain antibodies, ligand-binding domains, and enzymes have been successfully generated by this method.
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