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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
Published on: January 31, 2018
Multiple peak formation of avidin on an iminobiotin affinity support
1Department of Chemistry, Box 9573, Mississippi State University, Mississippi State, MS 39762, USA. TL45@ra.msstate.edu
Journal of Chromatographic Science
|March 17, 2004
Abstract:
When pure avidin is chromatographed on a homemade iminobiotin column, two distinct peaks are observed using a gradient solvent system starting with a pH 9 buffer. Proteins in both peaks are shown to be identical by nondenatured polyacrylamide gel electrophoretic experiment. The weak interaction of the protein with the stationary phase at pH 9 could cause this unusual behavior.

