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Related Experiment Videos

Uropathogenic Escherichia coli--a preliminary study.

N S Srikanth1, Ragini Macaden

  • 1Dept. of Microbiology, St. John's Medical College, Bangalore.

Indian Journal of Pathology & Microbiology
|March 19, 2004
PubMed
Summary

This study investigated urinary Escherichia coli, finding common O serotypes and virulence factors like hemolysin and Mannose resistant Haemagglutination (MRHA). Many E. coli strains showed hemolysin, while fewer were serum resistant or exhibited MRHA.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Urology

Background:

  • Escherichia coli (E. coli) is a common cause of urinary tract infections (UTIs).
  • Virulence factors such as haemolysin and adhesins contribute to E. coli pathogenicity.
  • Understanding serotype prevalence is crucial for epidemiological studies and vaccine development.

Purpose of the Study:

  • To characterize urinary isolates of E. coli.
  • To determine the prevalence of haemolysin, adhesins (specifically Mannose resistant Haemagglutination - MRHA), and serum resistance.
  • To identify common O serotypes among these isolates.

Main Methods:

  • Isolation and characterization of 144 urinary E. coli strains.
  • Assays for haemolysin production.

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  • Assessment of serum resistance.
  • Hemagglutination assays, specifically for Mannose resistant Haemagglutination (MRHA).
  • Serotyping to determine O antigen prevalence.

    • Main Results:

    • Out of 144 E. coli isolates, 72 (50%) produced haemolysin.
    • Seven isolates (4.9%) demonstrated resistance to the bactericidal effects of serum.
    • Fifty isolates (34.7%) exhibited Mannose resistant Haemagglutination (MRHA).
    • The most prevalent O serotypes identified were O101, O68, O04, and O25.

    Conclusions:

    • Urinary E. coli isolates frequently possess virulence factors like haemolysin and MRHA.
    • A significant proportion of E. coli strains exhibit resistance to serum.
    • Specific O serotypes (O101, O68, O04, O25) are common in urinary isolates, providing epidemiological insights.