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Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
Published on: April 11, 2018
[Crystalline nickel sulfide-induced genomic instability in transformed human broncho-epithelial cells]
Chuan-de Chen1, Zhong-liang Wu, Jia-kun Chen
1Center for Disease Control and Prevention of Luohu District, Shenzhen 518020, China.
Objective:
To detect the genomic instability in the 16 human broncho-epithelial (16HBE) cells induced by crystalline nickel sulfide so as to provide the scientific basis for further study of nickel-induced cancer molecular mechanism.
Methods:
To analyse the genomic instability in transformed 16HBE cells induced by crystalline nickel sulfide by random amplified polymorphic DNA (RAPD).
Results:
All the 7 random primers selected could amplify 1 - 6 clear PCR bands. There were no significant differences between transformed 16HBE cells and negative control cells in the 4th, 5th, and 7th primers, but in the rest 4 primers there were significant differences, with special PCR bands for the same primer, indicating that genomic instability in transformed 16 HBE cells was induced by crystalline nickel sulfide.
Conclusion:
Crystalline nickel sulfide could induce genomic instability in 16HBE cells.
