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Updated: Aug 25, 2026
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
[Construction and identification of yeast expression vectors containing human platelet-derived growth factor B-chain
Chun-lu Yang1, Jian-ting Chen, Fan Deng
1Department of Orthopedic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. ycl_2002@163.com
Objective:
To investigate the expression efficiency of human platelet-derived growth factor B-chain (PDGF-B) gene in yeast and assess the activity of the expressed product.
Methods:
A full-length complementary DNA of human PDGF-B gene was amplified from the total RNA extracted from human vascular endothelial cells using reverse transcription (RT)-PCR and then cloned into pGEM-T vector. The PCR products with specific primers were recombined into yeast expression plasmids pMETB or pMETalphaA, followed by identification with restriction endonuclease.
Results:
The 578 bp fragment encoding mature PDGF-BB peptide with signal peptide and the 340 bp fragment without signal peptide were identified and verified by restriction endonuclease mapping and sequencing, and two yeast expression vectors pMETB-PDGFB(1) and pMETalphaA-PDGFB(2) were reconstructed.
Conclusion:
The yeast expression vectors containing human PDGF-B gene have been successfully constructed for further studies of gene expression in yeast.
