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[Colorimetric micromethod for serum iodine determination]
P Houzé1, M Corvisier, M-E Toubert
1Laboratoire de biochimie A, Hôpital Saint-Louis, 1, avenue Claude Vellefaux, 75010 Paris. pascal.houze@sls.ap-hop-paris.fr
Annales De Biologie Clinique
|March 30, 2004
Summary
Measuring serum iodine offers a simpler alternative to urine tests for assessing iodine deficiency, especially in children. This new colorimetric method provides accurate results, aiding in the diagnosis of thyroid disorders.
Area of Science:
- Biochemistry
- Endocrinology
- Analytical Chemistry
Context:
- Iodine deficiency disorders (IDDs) are a global health concern.
- Urinary iodine concentration (UIC) is the gold standard for assessing population iodine status.
- Collecting 24-hour urine samples, particularly from children, presents significant practical challenges.
Purpose:
- To develop and validate a colorimetric method for serum iodine measurement.
- To overcome the limitations associated with 24-hour urine collection for iodine assessment.
- To provide a reliable alternative for evaluating iodine status in clinical settings.
Summary:
- A novel colorimetric assay was adapted for microtiter plates, optimizing serum mineralization.
- The method demonstrates linearity up to 2400 nmol/L with a detection limit of 75 nmol/L and precision below 10%.
- Validation against an automated technique confirmed the method's accuracy, showing good agreement.
Impact:
- This simple and validated serum iodine assay can serve as a valuable tool for diagnosing and monitoring iodine status.
- It offers a practical solution for assessing iodine levels, especially in pediatric populations.
- The method may contribute to better management and prevention strategies for thyroid dysfunction and related disorders.