Evaluating "fast" micellar monolithic liquid chromatography for high-throughput quantitative structure-retention...

Area of Science:

• Analytical Chemistry
• Chromatography
• Drug Discovery

Background:

• Micellar liquid chromatography (MLC) is used for high-throughput quantitative structure-retention relationships (QSRR).
• MLC estimates drug membrane permeability via the octanol-water partition coefficient (log P).
• Monolithic silica columns offer potential advantages in chromatographic speed.

Purpose of the Study:

• To evaluate monolithic silica columns for micellar liquid chromatography.
• To compare elution behavior on monolithic vs. particle-based columns for log P determination.
• To assess the impact of micellar mobile phases on retention characteristics.

Main Methods:

• Micellar liquid chromatography was performed using both monolithic and particle-based silica columns.
• Diverse basic pharmaceutical substances were analyzed.
• Elution behavior was studied with and without micellar mobile phases.
• Principal component analysis was employed to compare retention characteristics.

Main Results:

• Monolithic columns demonstrated effective performance in micellar liquid chromatography.
• Combining monolithic columns with micellar media resulted in faster log P estimations.
• The developed method showed potential for improved drug permeability predictions.

Conclusions:

• Monolithic silica columns are suitable for micellar liquid chromatography applications.
• The integration of monolithic columns and micellar media enhances the speed and accuracy of log P determination.
• This approach can accelerate drug discovery by providing rapid permeability insights.