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Surgical techniques for cell transplantation into the mouse cochlea
Fukuichiro Iguchi1, Takayuki Nakagawa, Ichiro Tateya
1Department of Otolaryngology--Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Acta Oto-Laryngologica. Supplementum
|April 14, 2004
Summary
Surgical approaches for inner ear cell transplantation were compared. The lateral semicircular canal (LSCC) approach safely delivered cells, preserving auditory function, while the cochlear lateral wall (CLW) approach caused hearing loss.
Area of Science:
- Otolaryngology
- Regenerative Medicine
- Neuroscience
Background:
- Cell transplantation holds promise for treating inner ear disorders.
- Developing safe and effective surgical methods is crucial for successful cell delivery.
Purpose of the Study:
- To compare two surgical approaches for transplanting neural stem cells into the mouse inner ear.
- To evaluate the distribution and survival of transplanted cells.
- To assess the impact of transplantation on auditory function.
Main Methods:
- Fetal mouse neural stem cells were transplanted into C57BL/6 mice via the lateral semicircular canal (LSCC) or cochlear lateral wall (CLW).
- Cell distribution was analyzed post-transplantation.
- Auditory brain stem responses (ABRs) were measured to assess hearing function.
Main Results:
- LSCC transplantation resulted in cell survival in the scala vestibuli and scala tympani with minimal hearing threshold elevation (<10 dB SPL).
- CLW transplantation led to significant hearing loss, despite cell engraftment in the scala media.
- LSCC approach facilitated cell delivery to perilymph without auditory impairment.
Conclusions:
- Cell transplantation via the LSCC is a viable method for delivering cells to the inner ear's perilymphatic space.
- The CLW approach, while targeting the endolymph, appears to induce auditory dysfunction.
- Surgical approach significantly influences cell distribution and functional outcomes in inner ear transplantation.