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Related Experiment Videos

The evolution of microarrayed compound screening.

Michael Hoever1, Peter Zbinden

  • 1Discovery Partners International AG, Gewerbestrasse 16, CH-4123 Allschwil, Switzerland. mhoever@discoverypartners.com

Drug Discovery Today
|April 15, 2004
PubMed
Summary

Microarrayed compound screening (microARCS) is now a robust ultra-high-throughput technology. Recent advancements streamline processes, showing excellent overlap with traditional methods and no compound bias.

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Use of FLIPR membrane potential dyes for validation of high-throughput screening with the FLIPR and microARCS technologies: identification of ion channel modulators acting on the GABA(A) receptor.

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Area of Science:

  • Drug discovery and development
  • Chemical biology
  • High-throughput screening technologies

Background:

  • Microarrayed compound screening (microARCS) is an emerging ultra-high-throughput screening technology.
  • Traditional screening methods face limitations in throughput and efficiency.
  • Advancements are crucial for optimizing microARCS for drug discovery.

Purpose of the Study:

  • To review recent developments in microarrayed compound screening (microARCS).
  • To highlight improvements making microARCS a robust and efficient ultra-high-throughput screening technology.
  • To assess the performance and applicability of microARCS in various screening projects.

Main Methods:

  • Improvements in compound spotting, including quality-control methods.

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  • Enhancements in gel casting and imaging techniques.
  • Development of automated software for image analysis and deconvolution.

    • Main Results:

    • Streamlined screening processes through technological advancements.
    • Successful conclusion of diverse cell-based and non-cell-based screening projects using microARCS.
    • Excellent overlap in identified hits when compared to standard microtitre-plate screening.
    • Absence of bias in compound identification based on logP values, despite dry-state solubilization.

    Conclusions:

    • MicroARCS has evolved into a powerful and reliable ultra-high-throughput screening platform.
    • The technology demonstrates comparable efficacy to traditional methods with enhanced efficiency.
    • MicroARCS is suitable for a wide range of screening applications without inherent compound selection bias.