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Expression of type IV collagen by Lewis pulmonary carcinoma cells
G P Gaenko1, S V Khaidukov, Yu G Molotkovskii
1M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Abstract:
Study of the expression of type IV collagen by Lewis pulmonary carcinoma cells (PLMG-T, PLMG-M1, PLMG-M2) showed that these cells produce type IV collagen. The expression of type IV collagen in PLMG-T and PLMG-M2 cells approximately 2-fold surpassed that in PLMG-M1 cells. The number of active cells (synthesizing type IV collagen) in PLMG-M1 culture was 1.6 and 2.2 times lower than in PLMG-M2 and PLMG-T cultures, respectively. It was found that cell culture medium modulated expression of type IV collagen and this effect was mediated via cell receptors.
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