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Updated: Aug 24, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
Posttranscriptional gene silencing in plants
Susan Varsha Wesley1, Chris Helliwell, Ming-Bo Wang
1CSIRO Plant Industry, Canberra, Australia.
Abstract:
Double-stranded RNA when introduced into cells results in severe reduction of the target mRNA. This phenomenon is known as posttranscriptional gene silencing in plants and RNA interference in animals. Hairpin RNA-mediated gene silencing exploits this cellular mechanism. A convenient way of generating hairpin constructs is to use generic vectors such as pHANNIBAL and pHELLSGATE, vectors based on the Gateway technology. These vectors are suitable for high-throughput gene silencing, and the silencing effect is stably inherited over many generations.
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